Protein Concentration Determination in Latex Glove Using Biocompatibility Morphological Mean Test

Main Article Content

Chean Khim Toa
Kok Swee Sim
Yee Kit Chan

Abstract

Latex gloves are seen as an indispensable item in the healthcare field because it offers superior protection for both the medical staff and patient against harmful substances. However, latex gloves with high protein concentration have a high possibility to induce latex allergy which in the worst case can lead to a life-threatening condition. To minimize the occurrence of an allergy reaction, the computerized Biocompatibility Morphological Mean (BMM) test for protein detection is proposed. This test initially goes through the chemical process to determine the protein that resides in the glove sample. After that, the sample is electronically converted into a digital image. Finally, the image undergoes color image processing for calculating the color difference values. These values are then plotted on a standard curve. A high correlation coefficient (R2>0.97) of the standard curve gives better accuracies. The proposed method only takes about 40 minutes to complete the test, while existing methods need at least 6 hours.


 


[Manuscript received: 17 Jun 2019| Accepted: 7 Aug 2019 | Published: 30 Oct 2020]

Article Details

How to Cite
Toa, C. K., Sim, K. S., & Chan, Y. K. (2020). Protein Concentration Determination in Latex Glove Using Biocompatibility Morphological Mean Test. International Journal on Robotics, Automation and Sciences, 2, 15–21. https://doi.org/10.33093/ijoras.2020.2.3
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Articles

References

K. P. Yong, K. S. Sim, H. Y. Ting, W. K. Lim, K. L. Mok, A. H. M. Yatim, “Latex Glove Protein Estimation Using Maximum Minimum Area Variation ," International Conference on Biomedical Engineering, vol. 35, April 2011.

M. Mansouri, M. Tidley, K. A. Sanati, and C. Roberts, “Comparison of blood transmission through latex and nitrile glove materials,” in National Center for Biotechnology Information, pp. 205–210, January 2010.

E. Yip and P. Cacioli, "The manufacture of gloves from natural rubber latex," Journal of Allergy and Clinical Immunology, vol. 110, 2002.

Canadian Agency for Drugs and Technologies in Health, "Disposable Gloves for Use in Healthcare Settings: A Review of the Clinical Effectiveness, Safety, Cost-Effectiveness, and Guidelines," in Canadian Agency for Drugs and Technologies in Health, 2011.

A. Oomman and S. Oomman, "Latex glove allergy: The story behind the "invention” of the surgical glove and the emergence of latex allergy," Archives of International Surgery, vol. 3, 2013.

G. Y. Yew, T. C. Tham, C. L. Law, D. T. Chu, C. Ogino, and P. L. Show, “Emerging crosslinking techniques for glove manufacturers with improved nitrile glove properties and reduced allergic risks,” Materials Today Communications, vol. 19, pp. 39–50, March 2019.

M. Amarasekera, N. Rathnamalala, S. Samaraweera, and M. Jinadasa, “Prevalence of latex allergy among healthcare workers,” International Journal of Occupational Medicine and Environmental Health, vol. 23, no. 4, pp. 391–396, 2010.

E. Yip, “Measurements of Total Extractable Proteins in Latex Gloves: A Comparative Study of the RRIM and ASTM Tests,” International Rubber Conference, vol. 12, no. 3, pp. 166–175, 1997.

K. S. Sim, F. S. Chin, C. P. Tso, and L. W. Thong, “Protein Identification in Latex Gloves for Bio-compatibility using Maximum Minimal Variation Test,” International Conference on Biomedical Engineering, pp. 611–614, 2008.

R. V. Nouroozi, M. V. Nouroozi, and M. Ahmadizadeh, “Determination of Protein Concentration Using Bradford Microplate Protein Quantification Assay,” International Electronic Journal of Medicine, vol. 4, no. 1, pp. 11–17, 2015.

E. V. Woodburn, K. D. Long, and B. Cunningham, "Analysis of Paper-Based Colorimetric Assays With a Smartphone Spectrometer," IEEE Sensors Journal, 2018.

H. Y. Ting, K. P. Yong, K. L. Mok, and K. S. Sim, “Downhill Search-Based Nrl Glove Protein Estimation,” Rubber Chemical Technolgy, vol. 86, no. 4, pp. 653–663, 2013.

H. Hasma, D. Dazylah, and M. N. Qamarina, “The Factor Contributing to Higher Extractable Protein Content in Natural Rubber Latex Glove as Determined by ASTM D5712-99 over ASTM D5712-95 and its Relation to Allergen Content,” Journal of Rubber Research, vol. 9, no. 2, pp. 73–77, 2006

A. Lucas, "Modification of the Lowry Method for Analysis of Soluble Latex Proteins," Journal of Toxicology Methods, vol. 10. 2008.

J. H. Waterborg and H. R. Matthews, “The lowry method for protein quantitation,” Methods in Molecular Biology, vol. 1, pp. 1–3, 1984.

J. D. Easterbrook, T. Shields, S. L. Klein, and G. E. Glass, “Smartphone for Point-of-Care Quantification of Protein by Bradford Assay Camila,” Journal of the Brazilian Chemical Society, vol. 22, no. 12, pp. 2396–2402, 2011.

N. Kruger, “The Bradford Method For Protein Quantitation,” in The Protein Protocols Handbook, pp. 15–21, 2002.

S. Bianco, F. Gasparini, A. Russo, and R. Schettini, "A New Method for RGB to XYZ Transformation Based on Pattern Search Optimization," IEEE Transactions on Consumer Electronics, vol. 53. 2007.

K. León, D. Mery, F. Pedreschi, and J. León, “Color measurement in L*a*b* units from RGB digital images,” Food Research International, vol. 39, no. 10, pp. 1084–1091, 2006.

M. A. Kriss, L. W. Macdonald, and M. D. Fairchild, "Colour Science using MATLAB," in Wiley-IS & T Series in Imaging Science and Technology, 2012.

ASTM D5712-15, “Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method,” 1999. [Online]. Available: www.astm.org.